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Life Sciences, Vol. 64, No. 11, pp895-904, 1999 Scientific task: Characterizing the effects of dexamethasone on ODC gene expression and enzyme activity in the lung of divulge pups. Facts Pertaining the Problem: Numerous studies have shown that gestational exposure to glucocorticoid increases the productions and forgive of surfactant and accelerates morphological development of the fetal lung, while intermission of the pituitary-adrenal bloc violates lung growth, as do repressors of glucocorticoid biosynthesis. Synthetic glucocorticoids are routinely administered to large(predicate) women at risk of premature delivery in an set about out to accelerate the functional maturation of the fetal lung and thereby issue the incidence of respiratory distress syndrome in the newborn, a star cause of neonatal death in pre-term infants. Glucocorticoids did non bowdlerize surfactant production when given postnatally. However, they were found to inhibit proliferation of lung electr ic cells and to impair formation of new alveoli, suggesting that endogenous glucocorticoids whitethorn modulate cell development in the postnatal lung. The Proposed doubtful Solution: repayable to its extremely short half-life in animal tissues, ODC is speedily induced or suppressed by agents which influence development.

The plan for the Experiment to Test the Hypothesis: squiffy CD female rats with litters of 10 pups were housed in breeding cages in a vivarium keep at 22 degrees Celsius with a 12-h light-dark cycle, and provided nutrition and water ad libitum. Animals were killed by decapitation and lungs and livers were quickly dissected, equate in 19 volumes of ice-cold 10mM Tris -HCl, centrifuged at 26,000 x g for 20 min, ! and the supported assayed for ODC activity by a qualifying of the method of Russell and Synder. good liver RNA was extracted with acid guanidinium thiocyanate-phenol-choloform fit to Chomczynsky and Sacchi. The Conclusion relate to the Objectives: The results from these studies demonstrate that neonatal lung ODC activity is highly sensitive... If you want to lead a full essay, order it on our website: OrderEssay.net

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