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Friday, June 14, 2013

Greek

Quiz 9 c alto conquerher into question Pool Below please give-up the ghost out the ninth of the every week lab essayzes. All 10 points of the quiz volition draw from this list of questions. 1.Describe in exuberant the hear you will do this week on the cellular fractions from last week. (5 points) 2.Fill in the pastime table so that all tubes admit a utmost volume of 60 μl: (5 points) Assay Tube #craved [BSA](mg/ml)Volume of 1 mg/mlBSA (μl)Volume of inorganic phosphate buffer (μl) 1 = waste0060 20.2 30.4 40.6 50.8 61.0 3.What does the Bradford confirmation prevention? (1 point) 4.Which dye does the Bradford assay physical exercise? (1 point) 5.To run into the total protein content in a event fraction, what moldiness(prenominal) you multiply by what? withdraw: there be 3 quantities! (3 points) 6.a. What dyes will we use for fluorescence microscopy & what do they stain? (4 points) b. In the slides, the potency of staining of _______________________________ is important. (1 point) 7.What is enzyme operation? (2 points) 8.What is the deduction of total activity? (3 points) 9.What is the logical implication of specific activity? (3 points) 10.In what units will we measure enzyme activity? (2 points) 11.Which 2 enzymes are we following & where is to each(prenominal) one make? (4 points) 12.a.
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With which shaft will we measure the fall of product form by the enzymatic chemical reactions? (1 point) b. What property must a message have in order to be measured by the factor in part (a)? (1 point) c. What is the measurable product of each enzyme reaction? (2 points) 13.For an enzymatic reaction, what should a dummy contain? Hint: the upshot is NOT “extra phosphate buffer”! (2 points) 14.What substances will you carry to each enzyme reaction to dispel it? What do these substances do? (5 points) 15.a. For succinate dehydrogenase, what volumes of enzyme go into the reactions? There are 2 different amounts, check to 2 different dilutions. (2 points) b. If...If you want to get a in just essay, order it on our website: Orderessay

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